Cellular and molecular basis of the host-pathogen relationships












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            * Cellular and molecular basis of the host-pathogen relationships
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            Research is focused on the characterization of mechanisms that participate in the transmis

            infectious agents, establishment/progress of infection in the host body, pathogenesis, and
            immune response. Virology research aims at the cell defense against infections caused by s
            (polyomavirus, papillomavirus, and hepatitis B virus) and HIV virus, particularly at the p

            with restriction of virus infection by mechanisms of innate immunity. These mechanisms are
            during the early phase of virus cell interaction, i.e., during the transport of virus part
            cell nucleus. Also, the mechanisms of cell defense evasion by viruses are analyzed. Charac

            microenvironment of infected cells and tumors of viral etiology and escape of these tumors
            reactions are also in focus. As for the most important results, epigenetic control via met

            promotor has been disclosed in HIV replication. Bacteriology research is focused on pore-f
            that modulate immune response. Also, secondary metabolites exhibiting diverse functions ar
            their influence on cell physiology including characterization of cytoskeleton, membraneous

            and energy metabolism. In cooperation with the Institute of Microbiology ASCR, adenylate c
            produced by Bordetella pertussis and having immunomodulatory effects is intensively studie
            protein. Also, the interaction of other pore-forming compounds with the membrane is studie

            colicins and natural or synthetic antibacterial molecules. Helminthology research deals ma
            avian schistosomes that may attack humans as accidental hosts and cause cercarial dermatit
            main research topics, the role of parasite peptidases and other enzymes in skin and tissue

            cellular and humoral immune response of the host, immune evasion of parasites, and pathoge
            with schistosome migration in the host tissues (neuropathogenicity of Trichobilharzia rege
            focus. Characterization of important parasite proteins is facilitated by recent genomic an

            projects. As for the most important results, characterization of penetration glands and pe
            schistosome larvae has been performed, and effector molecules of the host immune system re
            elimination of parasites have been disclosed.





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            * Selected outputs
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            • Verrier E. R., Yim S. A., Heydmann L., El Saghire H., Bach C., Turon-Lagot V., Mailly L.
              Lucifora J., Durantel D., Pessaux P., Manel N., Hirsch I., Zeisel M. B., Pochet N., Schu
              T. F. (2018): Hepatitis B virus evasion from cyclic guanosine monophosphate-adenosine mo

              synthase sensing in human hepatocytes. Hepatology 68:1695-1709
            • Trejbalová K., Kovářová D., Blažková J., Machala L., Jilich D., Weber J., Kučerová D., V
              Hirsch I., Hejnar J. (2016): Development of 5' LTR DNA methylation of latent HIV-1 provi

              models and in long-term-infected individuals. Clinical Epigenetics 8: 19
            • Seydlová G., Beranová J., Bíbová I., Dienstbier A., Držmíšek J., Mašín J., Fišer R., Kon
              Večerek B. (2017): The extent of the temperature-induced membrane remodeling in two clos

              Bordetella species reflects their adaptation to diverse environmental niches. Journal of
              Chemistry 292: 8048-8058
            • Dolejs?ova? T., Sokol A., Bosa?k J., S?majs D., Konopa?sek I., Mikušová G., Fišer R. (20

              from Shigella boydii forms voltage-dependent pores. Journal of Bacteriology (online firs
              JB.00493-19)
            • Horák P., Mikeš L., Lichtenbergová L., Skála V., Soldánová M., Brant S. V. (2015): Avian

              and outbreaks of cercarial dermatitis. Clinical Microbiology Reviews 28: 165-190
            • Leontovyč R., Young N., Korhonen P., Hall R., Bulantová J., Jeřábková V., Kašný M., Gass
              (2019): Molecular evidence for distinct modes of nutrient acquisition between visceral a

              schistosomes of birds. Scientific Reports 9: 1347