The regulation of cell, tissue and organ development in health and disease ****************************************************************************************** * The regulation of cell, tissue and organ development in health and disease ****************************************************************************************** The research is concentrated on cell biology of both plant and animal, including human cel biological processes and structures are analyzed. Important achievement in plant cell biol discovery and subsequential functional characterization of plant multiprotein complex exoc has crucial role in vesicle transport, exocytosis, cell growth and motility. In addition, of evolutionary conserved actin nucleators – FH2 proteins – on structure and dynamics of m cytoskeleton in plants was described. In animal and human cell biology, the attention was on cancer cell transformation and malignant progression, with emphasis on cell signaling, structures and mechanisms of cell motility. Important achievement was the elucidation of s of invasive protrusions of cancer cells – invadopodia – in a complex 3D environment. Other discoveries were related to amoeboid invasiveness of cancer cells (the discovery of primar invasiveness in cells of mesenchymal origin, demonstration of the importance of amoeboid i metastasis of sarcomas, identification of key signaling proteins). In relation to analysis in malignant progression the role of CAS protein in invasiveness, metastasis and mechanore and elucidated. In addition, the tyrosine phosphorylation within SH3 domains was identifie general negative regulatory mechanism of cell signalling. The research in the field of developmental biology is mainly concentrated on genetics and of Xenopus tropicalis and Xenopus laevis. Among most important achievements within this fi the construction of linkage and physical map of Xenopus Tropicalis, which subsequently ena identification of many genes, responsible for mutant phenotypes of interest for biomedical Another important achievement was the evolutional analysis of human X chromosome origin ba sequential and cytogenetic data from X. tropicalis. Very important results came from evo?d morphogenesis of head tissue/structures, which form the body plan of vertebrates and their plasticity is responsible for the origin of crucial evolutional innovations and adaptive c ****************************************************************************************** * Selected outputs ****************************************************************************************** • Fernandes M, Rosel D, Brábek J. Limits to Precision Cancer Medicine. N Engl J Med. 2017 • Žárský V, Kulich I, Fendrych M, Pečenková T.  Exocyst complexes  multiple functions in p secretory pathways. Curr Opin Plant  Biol (2013): 16(6): 726-33. • Cerny R, Cattell M, S.Spengler T, Bronner-Fraser M, Yu F, M.Medeiros D. Evidence for the cooption model of vertebrate jaw evolution. PNAS (2010): 107(40): 17262-17267.